Journal: Journal of Immunology Research

Article Title: Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors

doi: 10.1155/2019/6705949

Figure Lengend Snippet: Panel of antibodies for mass cytometry.

Article Snippet: 25 , CD278 (ICOS) , 143Nd , DX29 , Surface , Fluidigm.

Techniques: Cytometry, Staining

Journal: Journal of Translational Medicine

Article Title: Non-invasive imaging with ICOS-targeting monoclonal antibody for preclinical diagnosis of rheumatoid arthritis in a humanized mouse model

doi: 10.1186/s12967-024-05899-w

Figure Lengend Snippet: A Scheme of Cy7 NHS ester conjugation with anti-human ICOS monoclonal antibody (mAb). B The characterization of labeled antibody at different concentrations. Relative fluorescence unit (RFU). C Cell uptake of Cy7-ICOS mAb in PMA/Iono activated, blocked, and resting T cells in huPBMCs at 1-hour incubation ( n = 3). D MFI was measured to assess cellular uptake of the ICOS mAb labeled with Cy7 fluorophore at a concentration of 100nM. All values represent the mean ± SEM unless otherwise specified. One-way ANOVA and t test was conducted for statistical significance. ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns p > 0.05

Article Snippet: The supplier of the in vivo monoclonal antibody (BE0353, clone: C398.4 A) against human ICOS was BioXCell, located in West Lebanon, USA.

Techniques: Conjugation Assay, Labeling, Fluorescence, Incubation, Concentration Assay

Journal: Journal of Translational Medicine

Article Title: Non-invasive imaging with ICOS-targeting monoclonal antibody for preclinical diagnosis of rheumatoid arthritis in a humanized mouse model

doi: 10.1186/s12967-024-05899-w

Figure Lengend Snippet: A Reference of 4-view Regions of Interest (ROI) drawing was implemented, encompassing perspectives from the TOP, Bottom, Left, and Right orientations. B 4-view NIRF images at all time points (24 h, 48 h, 72 h, 96 h) between the huPBMC-AIA ( n = 6) and control groups ( n = 4). C X-ray and photographs images merge NIRF imaging following ICOS mAb-Cy7 injection at 6 h, 24 h, 48 h, 72 h and 96 h. D Fluorescence Intensity (FI) of four distinct views was systematically assessed at various time points. E ROI quantification of RP and the RP/LP ratio at all time points examined between huPBMC-AIA and control. F Correlation between paw thickness and RP/LP ratio in NIRF. Significant correlation was observed in both huPBMC-AIA and control group. All values represent the mean ± SEM unless otherwise specified. One-way ANOVA and t test was conducted for statistical significance. ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns p > 0.05

Article Snippet: The supplier of the in vivo monoclonal antibody (BE0353, clone: C398.4 A) against human ICOS was BioXCell, located in West Lebanon, USA.

Techniques: Control, Imaging, Injection, Fluorescence

Journal: Journal of Translational Medicine

Article Title: Non-invasive imaging with ICOS-targeting monoclonal antibody for preclinical diagnosis of rheumatoid arthritis in a humanized mouse model

doi: 10.1186/s12967-024-05899-w

Figure Lengend Snippet: A Ex vivo imaging in major organs (1 heart, 2 liver, 3 lung, 4 spleen, 5 kidney, 6 intestine, 7 right paw (RP), 8 left paw (LP), 9 bone, 10 muscle, 11 skin, and 12 blood) at day 7 right after the last in vivo NIRF scan (X-ray and photographs images). B Fluorescence intensity (FI) statistics were analyzed for both paw tissues and major organs following the intravenous administration of ICOS mAb labeled with Cy7 fluorophore at the 96 h time point. C Intra-group correlation analysis, the uptake and metabolism of the anti-human ICOS-Cy7 were evaluated. Row/column order determined by unsupervised hierarchical clustering. Side bars represent log10 (FI of organs) at 96 h. D Correlogram depicting r2 Pearson correlation between FI measured in the indicated ROI, compared with the log10 (fold-change RP/LP)at various time points. E Principal Component Analysis (PCA) involved the transformation of the data into principal components, enabling a comprehensive exploration of patterns and variations within the normalized ICOS NIRF signals. F ROC analysis showing sensitivity against 100%specificity for distinguishing the huPBMC-AIA from control group based on FI fold change imaged at 24 h and 96 h. All values represent the mean ± SEM unless otherwise specified. One-way ANOVA and t test was conducted for statistical significance. ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns p > 0.05

Article Snippet: The supplier of the in vivo monoclonal antibody (BE0353, clone: C398.4 A) against human ICOS was BioXCell, located in West Lebanon, USA.

Techniques: Ex Vivo, Imaging, In Vivo, Fluorescence, Labeling, Transformation Assay, Control

Journal: Cell Reports Medicine

Article Title: Response and recurrence correlates in individuals treated with neoadjuvant anti-PD-1 therapy for resectable oral cavity squamous cell carcinoma

doi: 10.1016/j.xcrm.2021.100411

Figure Lengend Snippet:

Article Snippet: ICOS (C398.4A) , Fluidigm , Cat# 3168024B, RRID: AB_2858237.

Techniques: Recombinant, Isolation, Mass Cytometry, Software

Journal: Cell reports

Article Title: Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease

doi: 10.1016/j.celrep.2019.11.098

Figure Lengend Snippet:

Article Snippet: CD278/ICOS, 148Nd , Fluidigm , Cat#3148019B; Clone C398.4A; RRID: AB_2756435.

Techniques: Recombinant, Staining, Activation Assay, Software

Journal: Journal for Immunotherapy of Cancer

Article Title: Immune isolation-enabled nanoencapsulation of donor T cells: a promising strategy for mitigating GVHD and treating AML in preclinical models

doi: 10.1136/jitc-2023-008663

Figure Lengend Snippet: Key resource table

Article Snippet: APC-conjugated anti-mouse CD278 (C398.4A) , BD Biosciences , Cat#565883; RRID: AB_2744479.

Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Proliferation Assay, Software

Journal: Cell reports

Article Title: Neonatal Rhesus Macaques Have Distinct Immune Cell Transcriptional Profiles following HIV Envelope Immunization

doi: 10.1016/j.celrep.2019.12.091

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Armenian Hamster anti-human ICOS BB515, Clone C398.4A , BD Biosciences , Cat# 565880; RRID:AB_2744480.

Techniques: Isolation, Negative Control, Mutagenesis, Recombinant, Software

Journal: Molecular Systems Biology

Article Title: Molecular profiling reveals features of clinical immunity and immunosuppression in asymptomatic P. falciparum malaria

doi: 10.15252/msb.202110824

Figure Lengend Snippet:

Article Snippet: 151Eu‐conjugated hamster anti‐human ICOS , Fluidigm , Clone DX29; Cat# 3151020B.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Marker, Infection, Purification, Flow Cytometry, Blocking Assay, Generated, Recombinant, Saline, Staining, Software, Cytometry